Corona virus Isolation and Culture

Virus culture must be in a laboratory with qualified Biosafety Level 3 (BSL-3) can be carried out. The process is briefly described as follows: fresh samples of the patients sputum, faeces, etc. is determined on Vero E6 cells for viral culture vaccinated. The cytopathic effect (CPE) is observed after 96 hours. Proof of the viral nucleic acid in the culture medium indicates a successful culture. Virus titre measurement: After the dilution of the virus component concentration by a factor of 10 in series, the TCIDSO is determined by the micro-Cytopathic; method. Otherwise, viral viability is determined by plaque forming (PFU).

Detection of antibodies in the Serum.